Step 1. Please select the prediction type

     • If the sgRNA already exists and just need to predict its efficiency, please select sgRNA prediction

     • If you want to design sgRNA for a specific target sequence, please select Target prediction

Step 2 Choose the input type

     • You can select Sequence input and type/paste one sequence or Enter-separated list of sequences

     • You can select File Input and upload a .txt or .fasta file

Download target example file